Part:BBa_K1537015

35S promoter+translation initiation optimized sequence for dicot+Mass translation enhancer

35S promoter

The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively.( Kuluev et al, 2010)

Translation initiation optimized sequence for dicot

We use the nucleotide sequence to enhance translation initiation(Samir V. Sawant et al 2001).

Mass translation enhancer

Insertion of “GCT TCC TCC” after the initiator codon ATG can augment downstream gene-expression in plants(So Nakagawa et al 2008).

References

Kuluev, B. R., Knyazev, A. V., Lebedev, P. Ya., Iljassowa, A. A. and Chemeris, A. V.(2010) Construction of Hybrid Promoters of Caulimoviruses and Analysis of Their Activity in Transgenic Plants. Russian Journal of Plant Physiology ,Vol. 57, No. 4, 582-589. So Nakagawa, Yoshihito Niimura, Takashi Gojobori,Hiroshi Tanaka and Kin-ichiro Miura(2008) Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Nucl. Acids Res. 36 (3): 861-871. Samir V. Sawant, Kanti Kiran, Pradhyumna K. Singh, and Rakesh Tuli(2001)Sequence Architecture Downstream of the Initiator Codon Enhances Gene Expression and Protein Stability in Plants. Plant Physiology 126(4) 1630-1636.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 66